P-76: Cytogenetic Investigation of Parthenogenetic Mouse Embryos Generated from In Vitro Activated Oocytes by Hydrostatic Pressure in The Presence of Calcium Ionophore and Ethanol

Authors

  • Alibakhshi R
  • Azadbakht M
  • Mirahadi N
Abstract:

Background: The advances in cytogenetic techniques during the last few years have permitted not only the study of large populations of wild and domestic animals, but also the detection of chromosome anomalies in embryos. Chromosomal abnormalities are the most common cause of embryonic and fetal mortality in mammals. Most reports of chromosome anomalies in parthenogenetic embryos describe numerical aberrations comprising aneuploidies, haploidy, polyploidy and mixoploidy. Hydrostatic pressure specifically affects centriole microtubules and that proximal microtubuletriplets are more resistant than distal microtubule doublets. The present study has been carried out to investigate the effects of hydrostatic pressure in the absence or presence of calcium ionophore and ethanol on improve of chromosomal complements in 2-cell parthenogenetic embryos. Materials and Methods: 6 to 8-week-old female NMRI mouse were superovulated by an injection of 10 IU of PMSG, followed by 10 IU HCG 48 hours later. Metaphase ΙΙ oocytes were collected from oviduct 14 hours after HCG injection. Oocytes transferred to T6 medium and randomly assigned to following groups: non- treatment (control), hydrostatic pressure exposure (treatment I), 5μM calcium ionophore exposure (treatment II), ethanol exposure (treatment III), 5μM calcium ionophore with hydrostatic pressure exposure (treatment IV) and ethanol 7% with hydrostatic pressure exposure (treatment V). Groups of activated oocytes were further treated with 5 mg/ml cytochalasin B for 4 hours. About 24-hour post oocytes activation, slides were prepared according to an ‘air drying’ technique and the chromosomal complement of 2-cell embryos was studied by giemsa staining. Results: Results indicated that in embryos treated by calcium ionophore and hydrostatic pressure observated the most incidence of normal chromosome (Diploidy and Tetraploidy) (84%) and fewest incidence of anormal chromosome (haploidy, triploidy and mixopliody) (16%) observed compared with control group and other treatments (p<0.05). Conclusion: It suggests that the exposure to hydrostatic pressure in the presence of calcium ionophore and ethanol can be improve chromosomal complements in 2-cell parthenogenetic embryos.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

P-229: Chromosomal Analysis of Parthenogenetic Mouse Embryos Generated from In Vitro Activated Oocytes by Hydrostatic Pressure and Ethanol and Cytochalasin B

Background: Studies of preimplantation stage embryos by classic cytogenetic techniques have limitations, starting with the need for good metaphase stage when only one third of all analyzed embryos may show good quality metaphases. The incidence of chromosome anomalies in embryos produced by in vitro procedures is generally higher than that of embryos formed in vivo. Pressure specifically affect...

full text

P-94: The Effect of Calcium Ionophore A23187 and Ethanol on Parthenogenetic Activation of Mouse Oocytes in Presence of Hydrostatic Pressure and Cy-tochalasin B

Background: Parthenogenetic activation of mammalian oocytes using artificial stimuli is commonly used in various reproductive bio-techniques. Calcium ionophore is known to elevate intracellular calcium levels in the cytoplasm of oocytes through the influx of calcium from extracellular spaces. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial ...

full text

P-75: The Effect of Hydrostatic Pressure in The Presence of Different Concentrations of Extracellular Calcium, Ethanol and Cytochalasin B on Parthenogenetic Activation of Mouse oocytes

Background: The parthenogenetic of oocytes is important in cloning research, as artificial activation of oocytes is an essential component of nucleus transfer protocols. Hydrostatic pressure can act as a mechanical stimulator that rearranges egg contents. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial increase observed at fertilization. In...

full text

P-70: The Effect of Hydrostatic Pressure on Parthenogenetic Activation of Mouse Oocytes Derived from In vitro Grown Ovarian Follicles

Background: Parthenogenesis is the production of an embryo from a female gamete in the absence of any contribution from a male gamete, with or without the eventual development into an adult. In vivo, mammalian parthenogenesis is a rare event. Parthenogenesis can be efficiently induced in vitro with a variety of mechanical, chemical, and electrical stimuli in several species. Hydrostatic pressur...

full text

P-97: Parthenogenetic Activation of Mouse Oocyte Using Calcium Ionophore in The Presence of Different Concentrations of Extracellular Calcium

Background: Parthenogenetic activation is a possible way to produce homogeneous embryos with the some ploidy. Probably such embryos could be used in other areas of biotechnology. Calcium signals are known as important regulators of oocyte activation. Extracellular calcium is required for initiation of meiotic resumption and development. Calcium ionophore A23187 is known to elevate intracellular...

full text

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 3

pages  65- 65

publication date 2013-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023